| 1. | In addition , the function of secreting protein by grb - ast signal peptide was also studied in this paper
此外，还探讨了grb - ast信号肽分泌蛋白的功能。 |
| 2. | Some bacteria also secrete proteins that are toxic for the host ， leading to mild effects such as diarrhea ， or to fatal effects
有些细菌还会分泌对寄主有毒的蛋白质导致轻度反应如腹泻，重则会致命。 |
| 3. | Further study showed that aii protein was a kind of non - secreted protein , and aii gene was located on the chromosome of b . thuringiensis or b . cereus
研究中还发现，苏云金芽胞杆菌aii蛋白是一种非分泌型蛋白。 |
| 4. | In this thesis , signal peptide analyse software and trans - menbrane helix analyse software were integrated in the research of mycobacterium tuberculosis ( mtb ) secreted proteins prediction
本研究尝试了利用信号肽预测软件和跨膜螺旋预测软件对结核杆菌全蛋白组进行分泌性蛋白的预测分析研究。 |
| 5. | We wish to find some new secreted proteins of mtb h37rv . these new secreted proteins may be useful in the discovery of protective antigen , immune taxonomy and new medicine targets
作为探索性研究，这些新的分泌性蛋白可能会在保护性抗原、免疫分型以及寻找新的药物靶点等研究中提供一些线索。 |
| 6. | The expression products of pel gene was analysis by sds - page and olive oil plate , and the result indicated that pel gene was functionally overexpressed in pichia pastoris and up to 95 % of the secreted protein
表达蛋白pel - gs分泌至培养基中，分子量约28kd ，与pel大小相近，占分泌蛋白的95 。 |
| 7. | Follistatin , a kind of secreted protein , is known to antagonise the function of several members of the tgf - beta ( transforming growth factor - beta ) superfamily including myostatin , the most powerful inhibitor of muscle growth characterised to date
卵泡抑素( follistatin )是一种分泌蛋白，它可以拮抗tgf - beta ( transforminggrowthfactor ，转化生长因子)超家族中几种成员的功能，包括myostatin 。 |
| 8. | After the induction of iptg , the extracellular proteins of pet22asg / bl21 ( de3 ) plyss showed blue when treated with gus staining solution ; but the extracellular proteins of ck : bl21 ( de3 ) plyss showed no color change with the same treat . these results verified the function of secreting protein by grb - ast signal peptide
将表达载体pet22asg导入表达宿主菌e . colibl21 ( de3 ) plyss ，经iptg诱导表达，其胞外蛋白以gus染色液处理显蓝色；而对照空宿主菌bl21 ( de3 ) plyss诱导表达后，经同样的处理，颜色无明显变化，这初步证实了ast信号肽具有一定的分泌蛋白的功能。 |
| 9. | The thesis has two parts , one is the prediction the other is the confirmation . in the first part all proteins of mtb h37rv were scaned in use of two bio - software ( signal ? and tmhmm ) to analyse signal peptide and trans - menbrane helix , and discovered 182 proteins are possible secreted proteins
预测部分利用了两个生物学软件( signalp 、 tmhmm )对结核杆菌全蛋白质组做信号肽分析和跨膜区分析，通过数据整理找到182个蛋白可能是分泌性蛋白，再经blastp对ncbi已收录的蛋白质库中所有序列进行相似性比对分析，发现这182个蛋白中有12个为结核杆菌所特有。 |
| 10. | Sds - page results showed that as to mut + recombinant highest yield was obtained after 4 days inducing and with the culture time prolonged it reduced . pokeweed antiviral protein gene expressed well when methanol concentration reached 10g / l . pokeweed antiviral protein obtained high yield in thin acidic culture medium ( ph6 . 0 - 6 . 4 ) and its quantity in total mass of secrete protein exceeded 30 %
Sds - page分析结果表明， mut ~ +组菌株在甲醇诱导第四天后pap在培养液中积累量达到最高水平，延长培养时间会导致产量下降；在10g / l的甲醇浓度诱导下， pap的表达量达到最高；培养基ph值在偏酸性条件下( 6 . 0 - 6 . 4 ) pap的表达量都维持在较高的水平。 |
